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Rise of stem cell therapies in aesthetics

      Abstract

      Stem cells have the ability to self-renew and differentiate into other cell types, which forms the foundation for their use in regenerative medicine and cosmetic dermatology. Adipose-derived stem cells have proven particularly attractive in aesthetics given their relative ease of collection and abundance. Stem cells have been employed for the treatment of androgenetic alopecia and skin rejuvenation with promising results, but their incorporation into cosmeceuticals is still in its infancy. Despite promising preclinical data and small clinical studies, additional randomized, controlled trials and standardization of treatment are needed to truly understand the place of stem cells in the aesthetics arena. We review the current literature and discuss current controversies and debates.
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      References

        • Wang JV
        • Schoenberg E
        • Rohrer T
        • Zachary CB
        • Saedi N.
        Stem cells in aesthetic dermatology: bioethical and professional obligations.
        Arch Dermatol Res. 2019; 311: 833-835
        • Zuk PA
        • Zhu M
        • Mizuno H
        • et al.
        Multilineage cells from human adipose tissue: implications for cell-based therapies.
        Tissue Eng. 2001; 7: 211-228
        • Kapur SK
        • Dos-Anjos Vilaboa S
        • Llull R
        • Katz AJ
        Adipose tissue and stem/progenitor cells: discovery and development.
        Clin Plast Surg. 2015; 42: 155-167
        • Pourang A
        • Rockwell H
        • Karimi K.
        New frontiers in skin rejuvenation, including stem cells and autologous therapies.
        Facial Plast Surg Clin North Am. 2020; 28: 101-117
        • Cheng KH
        • Kuo TL
        • Kuo KK
        • Hsiao CC.
        Human adipose-derived stem cells: isolation, characterization and current application in regeneration medicine.
        GMBHS. 2011; 3: 53-62
        • Raposio E
        • Caruana G
        • Petrella M
        • et al.
        A standardized method of isolating adipose-derived stem cells for clinical applications.
        Ann Plast Surg. 2016; 76: 124-126
        • Raposio E
        • Caruana G
        • Bonomini S
        • Libondi G.
        A novel and effective strategy for the isolation of adipose-derived stem cells: minimally manipulated adipose-derived stem cells for more rapid and safe stem cell therapy.
        Plast Reconstr Surg. 2014; 133: 1406-1409
        • Gimble JM
        • Katz AJ
        • Bunnell BA.
        Adipose-derived stem cells for regenerative medicine.
        Circ Res. 2007; 100: 1249-1260
        • Stefanis AJ
        • Groh T
        • Arenbergerova M
        • Arenberger P
        • Bauer PO.
        Stromal vascular fraction and its role in the management of alopecia: a review.
        J Clin Aesthet Dermatol. 2019; 12: 35-44
        • Tonnard P
        • Verpaele A
        • Peeters G
        • Hamdi M
        • Cornelissen M
        • Declercq H.
        Nanofat grafting: basic research and clinical applications.
        Plast Reconstr Surg. 2013; 132: 1017-1026
        • Kapur SK
        • Katz AJ.
        Review of the adipose derived stem cell secretome.
        Biochimie. 2013; 95: 2222-2228https://doi.org/10.1016/j.biochi.2013.06.001
        • Rehman J
        • Traktuev J
        • Li S
        • et al.
        Secretion of angiogenic and antiapoptotic factors by human adipose stromal cells.
        Circulation. 2004; 109: 1292-1298
        • Sasaki GH.
        Review of human hair follicle biology: dynamics of niches and stem cell regulation for possible therapeutic hair stimulation for plastic surgeons.
        Aesthetic Plast Surg. 2019; 43: 253-266
        • DelaRosa O
        • Lombardo E
        • Beraza A
        • et al.
        Requirement of IFN-gamma-mediated indoleamine 2,3-dioxygenase expression in the modulation of lymphocyte proliferation by human adipose-derived stem cells.
        Tissue Eng Part A. 2009; 15: 2795-2806
        • Shi D
        • Liao L
        • Zhang B
        • et al.
        Human adipose tissue-derived mesenchymal stem cells facilitate the immunosuppressive effect of cyclosporin A on T lymphocytes through jagged-1-mediated inhibition of NF-kappaB signaling.
        Exp Hematol. 2011; 39 (.e1): 214-224
        • Gonzalez MA
        • Gonzalez-Rey E
        • Rico L
        • Buscher D
        • Delgado M.
        Derived mesenchymal stem cells alleviate experimental colitis by inhibiting inflammatory and autoimmune responses.
        Gastroenterology. 2009; 136: 978-989
        • Lee MJ
        • Kim J
        • Kim MY
        • et al.
        Proteomic analysis of tumor necrosis factor-alpha-induced secretome of human adipose tissue-derived mesenchymal stem cells.
        J Proteome Res. 2010; 9: 1754-1762
        • Moon KM
        • Park YH
        • Lee JS
        • et al.
        The effect of secretory factors of adipose-derived stem cells on human keratinocytes.
        Int J Mol Sci. 2012; 13: 1239-1257
        • Heo SC
        • Jeon ES
        • Lee IH
        • Kim HS
        • Kim MB
        • Kim JH.
        Tumor necrosis factor-alpha-activated human adipose tissue-derived mesenchymal stem cells accelerate cutaneous wound healing through paracrine mechanisms.
        J Invest Dermatol. 2011; 131: 1559-1567
        • Fukuoka H
        • Narita K
        • Suga H.
        Hair regeneration therapy: application of adipose-derived stem cells.
        Curr Stem Cell Res Ther. 2017; 12: 531-534
        • Fukuoka H
        • Suga H
        • Narita K
        • Watanabe R
        • Shintani S.
        The latest advance in hair regeneration therapy using proteins secreted by adipose-derived stem cells.
        Am J Cosmetic Surg. 2012; 29: 273-282
        • Fukuoka H
        • Suga H.
        Hair regeneration treatment using adipose-derived stem cell conditioned medium: follow-up with trichograms.
        Eplasty. 2015; 15: e10
        • Shin H
        • Ryu HH
        • Kwon O
        • Park BS
        • Jo SJ.
        Clinical use of conditioned media of adipose tissue-derived stem cells in female pattern hair loss: a retrospective case series study.
        Int J Dermatol. 2015; 54: 730-735
        • Perez-Meza D
        • Ziering C
        • Sforza M
        • Krishnan G
        • Ball E
        • Daniels E.
        Hair follicle growth by stromal vascular fraction-enhanced adipose transplantation in baldness.
        Stem Cells Cloning. 2017; 10: 1-10
        • Gentile P
        • Scioli MG
        • Bielli A
        • Orlandi A
        • Cervelli V.
        Stem cells from human hair follicles: first mechanical isolation for immediate autologous clinical use in androgenetic alopecia and hair loss.
        Stem Cell Investig. 2017; 4: 58
        • Gentile P.
        Autologous cellular method using micrografts of human adipose tissue derived follicle stem cells in androgenic alopecia.
        Int J Mol Sci. 2019; 20: 3446
        • Gentile P
        • Scioli MG
        • Cervelli V
        • Orlandi A
        • Garcovich S.
        Autologous micrografts from scalp tissue: trichoscopic and long-term clinical evaluation in male and female androgenetic alopecia.
        Biomed Res Int. 2020; 20207397162
        • Elmaadawi IH
        • Mohamed BM
        • Ibrahim ZAS
        • et al.
        Stem cell therapy as a novel therapeutic intervention for resistant cases of alopecia areata and androgenetic alopecia.
        J Dermatolog Treat. 2018; 29: 431-440
        • Kim WS
        • Park SH
        • Ahn SJ
        • et al.
        Whitening effect of adipose-derived stem cells: a critical role of TGF-beta 1.
        Biol Pharm Bull. 2008; 31: 606-610
        • Kim WS
        • Park BS
        • Kim HK
        • et al.
        Evidence supporting antioxidant action of adipose-derived stem cells: protection of human dermal fibroblasts from oxidative stress.
        J Dermatol Sci. 2008; 49: 133-142
        • Seo KY
        • Kim DH
        • Lee SE
        • Yoon MS
        • Lee HJ.
        Skin rejuvenation by microneedle fractional radiofrequency and a human stem cell conditioned medium in Asian skin: a randomized controlled investigator blinded split-face study.
        J Cosmet Laser Ther. 2013; 15: 25-33
        • Lee HJ
        • Lee EG
        • Kang S
        • Sung JH
        • Chung HM
        • Kim DH.
        Efficacy of microneedling plus human stem cell conditioned medium for skin rejuvenation: a randomized, controlled, blinded split-face study.
        Ann Dermatol. 2014; 26: 584-591
        • Prakoeswa CRS
        • Pratiwi FD
        • Herwanto N
        • et al.
        The effects of amniotic membrane stem cell-conditioned medium on photoaging.
        J Dermatolog Treat. 2019; 30: 478-482
        • Zhou BR
        • Zhang T
        • Bin Jameel AA
        • et al.
        The efficacy of conditioned media of adipose-derived stem cells combined with ablative carbon dioxide fractional resurfacing for atrophic acne scars and skin rejuvenation.
        J Cosmet Laser Ther. 2016; 18: 138-148
        • Coleman SR
        • Katzel EB.
        Fat grafting for facial filling and regeneration.
        Clin Plast Surg. 2015; 42: 289-300
        • Marten TJ
        • Elyassnia D.
        Fat grafting in facial rejuvenation.
        Clin Plast Surg. 2015; 42: 219-252
        • Bernardini FP
        • Gennai A
        • Izzo L
        • et al.
        Superficial enhanced fluid fat injection (SEFFI) to correct volume defects and skin aging of the face and periocular region.
        Aesthet Surg J. 2015; 35: 504-515
        • Gennai A
        • Zambelli A
        • Repaci E
        • et al.
        Skin rejuvenation and volume enhancement with the micro superficial enhanced fluid fat injection (M-SEFFI) for skin aging of the periocular and perioral regions.
        Aesthet Surg J. 2017; 37: 14-23
        • Tonnard P
        • Verpaele A
        • Carvas M.
        Fat grafting for facial rejuvenation with nanofat grafts.
        Clin Plast Surg. 2020; 47: 53-62
        • Charles-de-Sá L
        • Gontijo-de-Amorim NF
        • Maeda Takiya C
        • et al.
        Antiaging treatment of the facial skin by fat graft and adipose-derived stem cells.
        Plast Reconstr Surg. 2015; 135: 999-1009
        • Amirkhani MA
        • Shoae-Hassani A
        • Soleimani M
        • Hejazi S
        • Ghalichi L
        • Nilforoushzadeh MA.
        Rejuvenation of facial skin and improvement in the dermal architecture by transplantation of autologous stromal vascular fraction: a clinical study.
        Bioimpacts. 2016; 6: 149-154
        • Liang ZJ
        • Lu X
        • Li DQ
        • et al.
        Precise intradermal injection of nanofat-derived stromal ells combined with platelet-rich fibrin improves the efficacy of facial skin rejuvenation.
        Cell Physiol Biochem. 2018; 47: 316-329
        • Menkes S
        • Luca M
        • Soldati G
        • Polla L.
        Subcutaneous injections of nanofat adipose-derived stem cell grafting in facial rejuvenation.
        Plast Reconstr Surg Glob Open. 2020; 8: e2550
        • Baumann L.
        How to use oral and topical cosmeceuticals to prevent and treat skin aging.
        Facial Plast Surg Clin North Am. 2018; 26: 407-413
        • Bos JD
        • Meinardi MM.
        The 500 Dalton rule for the skin penetration of chemical compounds and drugs.
        Exp Dermatol. 2000; 9: 165-169
        • Fabi S
        • Sundaram H.
        The potential of topical and injectable growth factors and cytokines for skin rejuvenation.
        Facial Plast Surg. 2014; 30: 157-171
        • Alhaddad M
        • Boen M
        • Wu DC
        • Goldman MP.
        Red deer umbilical cord lining mesenchymal stem cell extract cream for rejuvenation of the face.
        J Drugs Dermatol. 2019; 18: 363-366
        • Sunder S.
        Relevant topical skin care products for prevention and treatment of aging skin.
        Facial Plast Surg Clin North Am. 2019; 27: 413-418
        • Schmid D
        • Schurch C
        • Blum P
        • Zulli F.
        Plant stem cell extract for longevity of skin and hair.
        SOFW-Journal. 2008; 134: 30-35
        • Wild J.
        Overview of plant stem cells in cosmeceuticals.
        Plast Surg Nurs. 2014; 34: 148-149
        • Nohynek L
        • Bailey M
        • Tähtiharju J
        • et al.
        Cloudberry (Rubus chamaemorus) cell culture with bioactive substances: establishment and mass propagation for industrial use.
        Engineer Life Sci. 2014; 14: 667-675
        • Barbulova A
        • Apone F
        • Colucci G.
        Plant cell cultures as source of cosmetic active ingredients.
        Cosmetics. 2014; 1: 94-104
        • Tito A
        • Carola A
        • Bimonte M
        • et al.
        A tomato stem cell extract containing antioxidant compounds and metal chelating factors protects skin cells from heavy metal-induced damages.
        Int J Cosmet Sci. 2011; 33: 543-552
        • Sanz MT
        • Campos C
        • Milani M
        • et al.
        Biorevitalizing effect of a novel facial serum containing apple stem cell extract, pro-collagen lipopeptide, creatine, and urea on skin aging signs.
        J Cosmet Dermatol. 2016; 15: 24-30
        • Snippert HJ
        • Haegebarth A
        • Kasper M
        • et al.
        Lgr6 marks stem cells in the hair follicle that generate all cell lineages of the skin.
        Science. 2010; 327: 1385-1389
        • Lough DM
        • Yang M
        • Blum A
        • et al.
        Transplantation of the LGR61 epithelial stem cell into full-thickness cutaneous wounds results in enhanced healing, nascent hair follicle development, and augmentation of angiogenic analytes.
        Plast Reconstr Surg. 2014; 133: 579-590
        • Taub A
        • Bucay V
        • Keller G
        • Williams J
        • Mehregan D.
        Multi-center, double-blind, vehicle-controlled clinical trial of an alpha and beta defensin-containing anti-aging skin care regimen with clinical, histopathologic, immunohistochemical, photographic, and ultrasound evaluation.
        J Drugs Dermatol. 2018; 17: 426-441
        • Wang JV
        • Schoenberg E
        • Saedi N
        • Ibrahim O.
        Platelet-rich plasma, collagen peptides, and stem cells for cutaneous rejuvenation.
        J Clin Aesthet Dermatol. 2020; 13: 44-49
        • Zimmerlin L
        • Donnenberg AD
        • Rubin JP
        • Basse P
        • Landreneau RJ
        • Donnenberg VS.
        Regenerative therapy and cancer: in vitro and in vivo studies of the interaction between adipose-derived stem cells and breast cancer cells from clinical isolates.
        Tissue Eng Part A. 2011; 17: 93-106
        • Zhang Y
        • Daquinag A
        • Traktuev DO
        • et al.
        White adipose tissue cells are recruited by experimental tumors and promote cancer progression in mouse models.
        Cancer Res. 2009; 69: 5259-5266
        • Linkov F
        • Kokai L
        • Edwards R
        • et al.
        The role of adipose-derived stem cells in endometrial cancer proliferation.
        Scand J Clin Lab Invest Suppl. 2014; 244: 54-58
        • Kuhbier JW
        • Bucan V
        • Reimers K
        • et al.
        Observed changes in the morphology and phenotype of breast cancer cells in direct co-culture with adipose-derived stem cells.
        Plast Reconstr Surg. 2014; 134: 414-423
        • Zhu Y
        • Sun Z
        • Han Q
        • et al.
        Human mesenchymal stem cells inhibit cancer cell proliferation by secreting DKK-1.
        Leukemia. 2009; 23: 925-933
        • Qiao L
        • Xu ZL
        • Zhao TJ
        • et al.
        Dkk-1 secreted by mesenchymal stem cells inhibits growth of breast cancer cells via depression of Wnt signalling.
        Cancer Lett. 2008; 269: 67-77
        • Sun B
        • Roh KH
        • Park JR
        • et al.
        Therapeutic potential of mesenchymal stromal cells in a mouse breast cancer metastasis model.
        Cytotherapy. 2009; 11: 289-298
        • Cousin B
        • Ravet E
        • Poglio S
        • et al.
        Adult stromal cells derived from human adipose tissue provoke pancreatic cancer cell death both in vitro and in vivo.
        PLoS One. 2009; 4: e6278
        • Han C
        • Sun X
        • Liu L
        • et al.
        Exosomes and their therapeutic potentials of stem cells.
        Stem Cells Int. 2016; 20167653489
        • Wan W
        • McGinley L.
        ‘Miraculous’ stem cell therapy has sickened people in five states.
        The Washington Post. 2020; (Available at:) (February 27, 2019. Accessed March 24,)