Methods using the artificial amplification of nucleic acids or signals after hybridization
are a new advantage in the diagnosis of genital infections with Chlamydia trachomatis. Commercially available nucleic acid amplification (NAA) assays have provided laboratories
with powerful tools having a particular impact in the detection of genital chlamydial
and gonococcal infections. Compared with cell culture methodologies and other techniques,
NAA assays such as ligase chain reaction (LCR), polymerase chain reaction (PCR), and
transcription-mediated amplification (TMA) have shown a performance pattern with a
higher sensitivity for invasive and noninvasive sample types and a high specificity
when compared with culture. This means that enzyme immunoassays (EIAs) and immunofluorescence
techniques, used for many years for the routine diagnosis of genital chlamydial infections,
are now more and more substituted by NAA assays.
To read this article in full you will need to make a payment
Purchase one-time access:
Academic & Personal: 24 hour online accessCorporate R&D Professionals: 24 hour online accessOne-time access price info
- For academic or personal research use, select 'Academic and Personal'
- For corporate R&D use, select 'Corporate R&D Professionals'
Subscribe:
Subscribe to Clinics in DermatologyAlready a print subscriber? Claim online access
Already an online subscriber? Sign in
Register: Create an account
Institutional Access: Sign in to ScienceDirect
References
- Screening for chlamydial infections in women attending family planning clinics.West J Med. 1983; 138: 375-379
- Asymptomatic urethritis in men.Sex Transm Dis. 1986; 13: 163-165
- Detection of Chlamydia trachomatis infections in women by Amplicor PCR.J Clin Microbiol. 1996; 334: 995-998
- Diagnosis of Chlamydia trachomatis genitourinary infection in women by ligase chain reaction assay of urine.Lancet. 1995; 345: 213-216
- Comparison of DNA amplification methods for the detection of Chlamydia trachomatis in first-void urine from asymptomatic military recruits.Sex Transm Dis. 1996; 23: 97-102
- RNA amplification by nucleic acid sequence-based amplification with an internal standard enables reliable detection of Chlamydia trachomatis in cervical scrapings and urine samples.J Clin Microbiol. 1996; 34: 3108-3114
- Performance of the Gen-Probe Amplified Chlamydia trachomatis assay in detecting Chlamydia trachomatis in endocervical and urine specimens from women and urethral and urine specimens from men attending sexually transmitted disease and family planning clinics.J Clin Microbiol. 1998; 36: 3230-3233
- Performance of transcription-mediated amplification and ligase chain reaction assays for detection of chlamydial infection in urogenital samples obtained by invasive and noninvasive methods.J Clin Microbiol. 1998; 36: 2666-2670
- Detection of Chlamydia trachomatis and Neisseria gonorrhoeae by enzyme immunoassay, culture, and three nucleic acid amplification tests.J Clin Microbiol. 2001; 39: 1751-1756
- Multicenter evaluation of the BDProbeTec ET system for detection of Chlamydia trachomatis and Neisseria gonorrhoeae in urine specimens, female endocervical swabs, and male urethral swabs.J Clin Microbiol. 2001; 39: 1008-1016
- Comparison of urethral swabs, urine, and urinary sediment for the isolation of Chlamydia.J Clin Microbiol. 1975; 2: 111-123
- Vulval swabs as alternative specimens for ligase chain reaction detection of genital chlamydial infection in women.J Clin Microbiol. 1997; 35: 836-838
- Comparison among performances of a ligase chain reaction-based assay and two enzyme immunoassays in detecting Chlamydia trachomatis in urine specimens from men with nongonococcal urethritis.J Clin Microbiol. 1996; 34: 1708-1710
- Comparison of manual amplicor PCR, Cobas Amplicor PCR, and LCx assays for detection of Chlamydia trachomatis infection in women by using urine specimens.J Clin Microbiol. 1997; 35: 402-405
- Diagnosis of genitourinary Chlamydia trachomatis infections using the ligase chain reaction on patient-obtained vaginal swabs.J Clin Microbiol. 1997; 35: 2133-2135
- Diagnosis of Neisseria gonorrhoeae infections in women by using the ligase chain reaction on patient-obtained vaginal swabs.J Clin Microbiol. 1997; 35: 2129-2132
- Criteria for selective screening for Chlamydia trachomatis infection in women attending family planning clinics.JAMA. 1986; 255: 1730-1734
- Urine-based screening for Chlamydia trachomatis in men attending sexually transmitted disease clinics.Sex Transm Dis. 2001; 28: 219-225
- Community-based urine screening for Chlamydia trachomatis with a ligase chain reaction assays.Ann Intern Med. 1997; 127: 796-803
- Urine screening for gonococcal and chlamydial infections at community-based organizations in a high-morbidity area.Sex Transm Dis. 2000; 27: 146-151
- Use of ligase chain reaction with urine versus cervical culture for detection of Chlamydia trachomatis in an asymptomatic military population of pregnant and nonpregnant females attending Papanicolaou smear clinics.J Clin Microbiol. 1998; 36: 1300-1304
- Sensitivity of ligase chain reaction assay of urine from pregnant women for Chlamydia trachomatis.Lancet. 1997; : 329-330
- Detection of genitourinary tract Chlamydia trachomatis infection in pregnant women by ligase chain reaction assay.Obstet Gynecol. 1997; 89: 556-560
- Seeking medical care for a sexually transmitted disease.Psychol Health. 1993; 8: 17-32
- The rapid test paradox.Sex Transm Dis. 1999; 26: 232-240
- Diagnosis of Chlamydia trachomatis endocervical infections by a commercial polymerase chain reaction assay.J Clin Microbiol. 1993; 31: 3023-3027
- Diagnosis of infection in asymptomatic men and women by PCR assay.J Clin Microbiol. 1996; 34: 1396-1400
- Reproducibility problems with the Amplicor PCR Chlamydia trachomatis test.J Clin Microbiol. 1997; 35: 957-959
- Reproducibility problems with the Abbott Laboratories LCx assay for Chlamydia trachomatis and Neisseria gonorrhoeae.J Clin Microbiol. 2000; 38: 2416-2418
- Influence of endocervical specimen adequacy on PCR and direct fluorescent-antibody staining for detection of Chlamydia trachomatis infection.J Clin Microbiol. 1997; 35: 3078-3081
- Improved PCR detection of Chlamydia trachomatis by using an altered method of specimen transport and high quality endocervical specimen.J Clin Microbiol. 1995; 33: 2265-2267
- Use of a single swab in multi-microbe or Flex Trans Transport Medium for detection of Chlamydia trachomatis by Roche Amplicor PCR and culture in specimens from two different patient population.J Clin Microbiol. 1997; 35: 2427-2429
- Population-based screening programs for Chlamydia trachomatis.Lancet. 1998; 349: 1070-1071
- Urine specimens from pregnant and nonpregnant women inhibitory to amplification of Chlamydia trachomatis nucleic acid by PCR, ligase chain reaction, and transcription mediated amplification.J Clin Microbiol. 1998; 36: 3122-3126
- Evaluation of the Abbott LCx Ligase chain reaction assay for detection of Chlamydia trachomatis and Neisseria gonorrhoeae in urine and genital swab specimens from a sexually transmitted disease clinic population.J Clin Microbiol. 1998; 36: 1630-1633
- Detection of PCR inhibitors in cervical specimens by using the Amplicor Chlamydia trachomatis assay.J Clin Microbiol. 1996; 34: 3072-3074
Article info
Identification
Copyright
© 2002 Elsevier Science Inc. Published by Elsevier Inc. All rights reserved.
