Research Article| Volume 6, ISSUE 4, P152-158, October 1988

Effects of minoxidil on human skin fibroblasts in culture

  • Sheldon R. Pinnell
    Address for correspondence: Sheldon R. Pinnell, MD, Department of Medicine, Division of Dermatology, Duke University Medical School, Durham, NC 27710.
    From the Department of Medicine, Division of Dermatology, Duke University Medical Center, Durham, North Carolina USA
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  • Saood Murad
    From the Department of Medicine, Division of Dermatology, Duke University Medical Center, Durham, North Carolina USA
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      Collagens are a family of extracellular proteins that have evolved to serve many specialized biologic functions. Fifteen collagen types with at least 18 genetically distinct polypeptide chains have been identified.1 In skin, eight of these collagens make up a major portion of the extracellular matrix including specialized structures such as basement membrane (Type IV collagen) and anchoring fibrils (Type VII collagen). All collagens are synthesized as procollagen precursor molecules. In addition to synthesis and precise association and alignment of the three polypeptide chains, biosynthesis involves many posttranslational modifications.2,3 These include synthesis of hydroxyproline catalyzed by prolyl hydroxylase and synthesis of hydroxylysine catalyzed by lysyl hydroxylase. Hydroxyproline is essential for maximum helical stability, and hydroxylysine participates in collagen cross-link formation as well as synthesis of specific glycosides catalyzed by collagen glycosyl transferases. Other enzymes incorporate into the procollagen molecule complex carbohydrate residues typical of glycoproteins. Following secretion from the cell, amino and carboxyterminal propeptides of the procollagen molecule are cleaved by separate enzymes. Certain lysyl and hydroxylysyl residues are then modified by lysyl oxidase to corresponding aldehydes, which interact with certain hydroxylysine residues to form intermodular cross-links.
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        • Burgeson RE
        • Morris NP
        The collagen family of proteins.
        in: Uitto J Perejda AJ Connective Tissue Disease—Molecular Pathology of the Extracellular Matrix. Dekker, New York1987: 3-28
        • Prockop DJ
        • Kivirikko KI
        Heritable diseases of collagen.
        N Engl J Med. 1984; 311: 376-386
        • Pinnell SR
        • Murad S
        Disorders of collagen.
        in: Standbury JB Wyngaarden JB Fredrickson DS The Metabolic Basis of Inherited Disease. 5th ed. McGraw-Hill, New York1982: 1425-1449
        • Murad S
        • Tajima S
        • Pinnell SR
        A paradoxical effect of hydralazine on prolyl and lysyl hydroxylase activities in cultured human skin fibroblasts.
        Arch Biochem Biophys. 1985; 241: 356-363
        • Murad S
        • Clayton J
        • Pinnell SR
        Selective depression of lysyl hydroxylase activity in human skin fibroblasts by minoxidil.
        Clin Res. 1986; 34: 770A
        • Murad S
        • Pinnell SR
        Suppression of fibroblast proliferation and lysyl hydroxylase activity by minoxidil.
        J Biol Chem. 1987; 262: 11973-11978
        • Pinnell SR
        • Murad S
        Effects of minoxidil on cultured human skin fibroblasts.
        Dermatologica. 1987; 175: 12-18
        • Murad S
        • Sivarajah A
        • Pinnell SR
        Serum stimulation of lysyl hydroxylase activity in cultured human skin fibroblasts.
        Connect Tissue Res. 1985; 13: 181-186
        • Cohen RL
        • Cook B
        • Weiss VC
        • et al.
        Mitogenic and immune suppressive effects of minoxidil on epidermal cells and lymphocytes in vitro.
        Clin Res. 1983; 31: 561A
        • Galbraith GMP
        • Thiers BH
        In vitro suppression of human lymphocyte activity by minoxidil.
        Int J dermatol. 1985; 24: 249-251
        • Baden HP
        • Kubilus J
        Effect of minoxidil on cultured keratinocytes.
        J Invest dermatol. 1983; 81: 558-560
        • Cohen RL
        • Alves MEAF
        • Weiss VC
        • et al.
        Direct effects of minoxidil on epidermal cells in culture.
        J Invest dermatol. 1984; 82: 90-93