Research Article| Volume 6, ISSUE 4, P42-45, October 1988

Mouse hair keratin cDNA: Implications and sequences

  • Arthur P. Bertolino
    Address for correspondence: Arthur P. Bertolino, MD, PhD, Assistant Professor of Dermatology, NYU Medical Center, 550 First Avenue, New York, NY 10016.
    From the Department of Dermatology, New York University Medical Center, New York, NY USA
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      An efficient method is described for the isolation of mouse hair follicle mRNAs. The RNA contains two peaks of mRNA activity, 11s and 18s. The larger-sized group of mRNAs encodes keratins of 46K, 47K, 58K, 59K, and 63K Mr. cDNAs were prepared using the follicular mRNAs as templates and cloned to produce a library. Initial screening of this library has identified five acidic and six basic mouse hair keratin clones.
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