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Abstract
In fundamental studies of cellular differentiation and gene expression in relation
to hair growth, there is a major need for a follicle culture system that would allow,
for example, the transfection of hair cells with isolated keratin genes so that their
tissue-specific expression can be investigated. Furthermore, such a culture system
would be of enormous benefit for examining the effects of nutritional factors, hormones,
and drugs known to alter hair growth as well as the biologic and molecular action
of carcinogens in the development of epithelial neoplasms.
Recently, there have been several attempts to develop follicle cultures, but with
only partial success.1,2 In our study, we used a collagen matrix so that isolated hair follicles could be
maintained as threedimensional structures for relatively prolonged periods of time,
thereby allowing analysis of the different facets mentioned above. The most definitive
feature for evaluating maintenance of hair growth is the continued synthesis of hair-specific
proteins.
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References
- In vitro growth and differentiation of epithelial cells derived from post-embryonic hair follicles.Aust J Biol Sci. 1982; 35: 103-109
- A method for culturing human hair follicle cells.Br J Dermatol. 1981; 104: 1-5
- Epidermal cell culture.in: 4th ed. Transplant Proc. 12. 1980: 114-122
- Collagen gel culture system and analysis of estrogen effects on mammary carcinogenesis.Arch Toxicol. 1984; 55: 91-96
- The differentiation of hair follicles and hairs in organ culture.Adv Biol Skin. 1969; 9: 35-60
- Induction of hair growth by implantation of cultured dermal papilla cells.Nature. 1984; 311: 560-562
- Cultivation of murine hair follicles as organoids in a collagen matrix.J Invest Dermatol. 1987; 89: 369-379
Article info
Footnotes
☆Supported in part by an Eleanor Roosevelt International Cancer Fellowship awarded to Dr. Rogers by the International Union Against Cancer and the American Cancer Society.
Identification
Copyright
© 1988 Published by Elsevier Inc.
